Issue 4, 2013
From the journal:

Chemical Communications

Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system†

Michael J. Gattner,a   Milan Vrabela  and  Thomas Carell*a  

* Corresponding authors

a Center for Integrated Protein Science at the Department of Chemistry, Ludwig-Maximilians-Universität, Munich, Butenandtstraße 5-13, Munich, Germany
E-mail: Thomas.Carell@cup.uni-muenchen.de
Fax: +49 (0)89 2180 77756
Tel: +49 (0)89 2180 77750

Abstract

Recently new lysine modifications were detected in histones and other proteins. Using the pyrrolysine amber suppression system we genetically inserted three of the new amino acids ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine site specifically into histone H3. The lysine at position 9 (H3 K9), which is known to be highly modified in chromatin, was replaced by these unnatural amino acids.

Graphical abstract: Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2CC37836A
Article type
Communication
Submitted
29 Oct 2012
Accepted
14 Nov 2012
First published
15 Nov 2012

Chem. Commun., 2013,49, 379-381

Permissions

Request permissions

Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system

M. J. Gattner, M. Vrabel and T. Carell, Chem. Commun., 2013, 49, 379 DOI: 10.1039/C2CC37836A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements