Issue 63, 2018

Magnetic-propelled Fe3O4–chitosan carriers enhance l-asparaginase catalytic activity: a promising strategy for enzyme immobilization

Abstract

Magnetic-propelled carriers comprising magnetic Fe3O4–chitosan nanoparticles were immobilized with L-asparaginase (L-ASNase). The enzyme displayed enhanced catalytic activity in a weak magnetic field, and thermal and pH stabilities. The conjugated L-ASNase presented higher thermostability and wider range of pH stability in comparison with those of free L-ASNase. Moreover, the reusability of conjugated L-ASNase significantly improved after immobilization and it retained 60.5% of its initial activity after undergoing 16 cycles. The conjugated L-ASNase maintained more than 50% and 48% initial activity after 4 weeks of storage at 4 °C and room temperature, respectively. Furthermore, we reveal that the activity of conjugated L-ASNase onto magnetic Fe3O4–chitosan particles increased by about 3-fold in the weak magnetic field at certain frequencies and flux density compared with that of free L-ASNase. Considering these excellent attributes, the magnetic-propelled mechanism in the transporting and activation of L-ASNase can be used by enhancing the catalytic activity, stability, and efficiency in vital implications for medicinal biotechnology.

Graphical abstract: Magnetic-propelled Fe3O4–chitosan carriers enhance l-asparaginase catalytic activity: a promising strategy for enzyme immobilization

Article information

Article type
Paper
Submitted
27 iyl 2018
Accepted
06 okt 2018
First published
23 okt 2018
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2018,8, 36063-36075

Magnetic-propelled Fe3O4–chitosan carriers enhance L-asparaginase catalytic activity: a promising strategy for enzyme immobilization

B. Ates, A. Ulu, S. Köytepe, S. A. Ali Noma, V. S. Kolat and T. Izgi, RSC Adv., 2018, 8, 36063 DOI: 10.1039/C8RA06346J

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