Issue 9, 2019

A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity

Abstract

We have developed an equipment-free biosensor for visual pesticide residue (acetamiprid) detection with high sensitivity and selectivity. The target acetamiprid associates with an aptamer sequence and releases trigger DNA to initiate the cyclic signal amplification process. An ingeniously designed hairpin DNA probe was employed as the sensing element, which contains the same sequence as the trigger DNA in the stem part of the hairpin and a G-rich sequence in the loop part of the hairpin. An exonuclease III (Exo III)-mediated target recycling strategy was utilized to achieve quadratic amplification. This two-step cyclic signal amplification results in the generation of numerous G-quadruplex DNAzymes that can catalyze the H2O2-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a colored signal readout, thus providing the amplified colorimetric detection of the target. This assay is ultrasensitive, enabling the visual detection of acetamiprid at concentrations as low as 10 pM without instrumentation. Our proposed sensing system also displays high selectivity and can be applied to determine acetamiprid residues in food samples. Moreover, the assay does not involve any chemical modification of DNA, which is simple and low-cost. This sensing platform provides a promising approach for on-site detection of target molecules in resource-constrained regions because of the easy and straightforward readout of results without the use of sophisticated apparatus.

Graphical abstract: A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity

Supplementary files

Article information

Article type
Paper
Submitted
19 noy 2018
Accepted
25 yan 2019
First published
25 yan 2019

Anal. Methods, 2019,11, 1168-1173

A target-induced and equipment-free biosensor for amplified visual detection of pesticide acetamiprid with high sensitivity and selectivity

L. Zeng, D. Zhou, J. Wu, C. Liu and J. Chen, Anal. Methods, 2019, 11, 1168 DOI: 10.1039/C8AY02513D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements