Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion†
Coline
Jumeaux, 
‡a
Eunjung
Kim,
‡a
Philip D.
Howes,
§a
Hyemin
Kim,
a
Rona
Chandrawati
¶a
and
Molly M.
Stevens
*a
‡a
Eunjung
Kim,
‡a
Philip D.
Howes,
§a
Hyemin
Kim,
a
Rona
Chandrawati
¶a
and
Molly M.
Stevens
*a
Abstract
We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.
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