Issue 16, 2020

A paper-based SERS assay for sensitive duplex cytokine detection towards the atherosclerosis-associated disease diagnosis

Abstract

Atherosclerosis (AS) is the most common factor causing many cardiovascular and cerebrovascular diseases and has received considerable attention. The occurrence mechanism of AS is uncertain because it is a choronically pathological process that is influenced by multi-aspects, among which cytokines play the key roles in regulating the processes of the immune system. For example, two key cytokines, namely, IL-10 and MCP-1 (chemokine), which are involved in AS progression with varied levels, can be used for AS status monitoring and early diagnosis of AS-associated diseases. Hence, a new paper-based, surface-enhanced Raman spectroscopy (SERS) sensing platform was established for the detection of these two key cytokines. By combining a nanoporous networking membrane as the substrate and SERS nanotags as the probe for signal reading, together with a sandwich design, sensitive and specific identification and quantification of cytokine targets in human serum were achieved with excellent sensing characteristics. The lowest detectable concentration was determined to be 0.1 pg mL−1 for both IL-10 and MCP-1 in human serum. The assay also exhibits high specificity towards target cytokine detection, with low-nonspecific binding and acceptable cross-reactivity in the presence of other structurally similar targets. Finally, the practicability was validated by performing duplex detection in human serum, which further demonstrates the high specificity of the assay for the detection of target cytokines. Taken together, these promising results illustrate that this developed sensing assay is a candidate for clinical multi-target analysis in real environments.

Graphical abstract: A paper-based SERS assay for sensitive duplex cytokine detection towards the atherosclerosis-associated disease diagnosis

  • This article is part of the themed collection: Biosensors

Supplementary files

Article information

Article type
Paper
Submitted
04 noy 2019
Accepted
05 dek 2019
First published
06 dek 2019

J. Mater. Chem. B, 2020,8, 3582-3589

A paper-based SERS assay for sensitive duplex cytokine detection towards the atherosclerosis-associated disease diagnosis

C. Li, Y. Liu, X. Zhou and Y. Wang, J. Mater. Chem. B, 2020, 8, 3582 DOI: 10.1039/C9TB02469G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements