Issue 21, 2021

Improved biocatalytic cascade conversion of CO2 to methanol by enzymes Co-immobilized in tailored siliceous mesostructured cellular foams

Abstract

CO2 can be enzymatically reduced to methanol in a cascade reaction involving three enzymes: formate-, formaldehyde- and alcohol dehydrogenase (FateDH, FaldDH, ADH). We report an improvement in the yield of this reaction by co-immobilizing the three dehydrogenases in siliceous mesostructured cellular foams (MCF). This material consists of large mesopores suitable for the co-immobilization of these comparatively large enzymes. To improve the interaction between the enzymes and support, the host silica material was functionalized with mercaptopropyl groups (MCF-MP). The enzymes were fluorescently labelled to independently monitor their uptake and spatial distribution into the particle. The three dehydrogenases were co-immobilized using two sequential methods. In the first one, the enzymes were immobilized according to the reaction order (FateDH → FaldDH → ADH) and secondly in order of increasing enzyme size (FateDH → ADH → FaldDH). Two protein loadings were also tested: 50 and 150 mgenzymes gsupport−1. We could observe a 4.5-fold higher methanol yield in comparison to enzymes free in solution when the enzymes were immobilized in order of size and with a loading of 50 mgenzymes gsupport−1. The results of this work show that by using MCF-MP, a simple method of immobilization can be applied to significantly increase the activity of the enzymes for the cascade reaction.

Graphical abstract: Improved biocatalytic cascade conversion of CO2 to methanol by enzymes Co-immobilized in tailored siliceous mesostructured cellular foams

Supplementary files

Article information

Article type
Communication
Submitted
28 iyl 2021
Accepted
03 okt 2021
First published
13 okt 2021
This article is Open Access
Creative Commons BY-NC license

Catal. Sci. Technol., 2021,11, 6952-6959

Improved biocatalytic cascade conversion of CO2 to methanol by enzymes Co-immobilized in tailored siliceous mesostructured cellular foams

M. Zezzi do Valle Gomes, G. Masdeu, P. Eiring, A. Kuhlemann, M. Sauer, B. Åkerman and A. E. C. Palmqvist, Catal. Sci. Technol., 2021, 11, 6952 DOI: 10.1039/D1CY01354H

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