Sensitive and specific detection of peroxynitrite and in vivo imaging of inflammation by a “simple” AIE bioprobe

Huilin Xie; Jingtian Zhang; Chao Chen; Feiyi Sun; Haixiang Liu; Xinyuan He; Kristy W. K. Lam; Zhao Li; Jacky W. Y. Lam; Guo-Qiang Zhang; Dan Ding; Ryan T. K. Kwok; Ben Zhong Tang

doi:10.1039/D0QM01004A

Sensitive and specific detection of peroxynitrite and in vivo imaging of inflammation by a “simple” AIE bioprobe†

Huilin Xie,

‡^a Jingtian Zhang,‡^b Chao Chen,^ab Feiyi Sun,^a Haixiang Liu,^c Xinyuan He,^a Kristy W. K. Lam,^a Zhao Li,

^a Jacky W. Y. Lam,^ac Guo-Qiang Zhang,*^b Dan Ding,

^b Ryan T. K. Kwok*^ac and Ben Zhong Tang

*^acde

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* Corresponding authors

^a Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China
E-mail: tangbenz@ust.hk

^b Key Laboratory of Bioactive Materials, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Ministry of Education, and College of Life Sciences, Nankai University, Tianjin 300071, China

^c HKUST Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area Hi-Tech Park, Nanshan, Shenzhen 518057, China

^d SCUT-HKUST Joint Research Institute, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China

^e AIE Institute, Guangzhou Development District, Huangpu, Guangzhou 510530, China

Abstract

A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO⁻) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage of phenylboronic moieties by ONOO⁻. TPE-DMAB can be facilely synthesized and displays excellent specificity and sensitivity towards ONOO⁻ (detection limit of 54 nM). Based on these novel properties of TPE-DMAB, we further produced its encapsulated nanoparticles and successfully used them to detect endogenous ONOO⁻ in macrophage cells and visualize the inflammation sites of mice, verifying the great potential of TPE-DMAB for biological applications.

This article is part of the themed collections: FOCUS: Recent progress on bioimaging technologies, FOCUS: Recent progress on aggregation-induced emission, 2021 Materials Chemistry Frontiers HOT articles and Luminogenic bioprobes for personal health technologies

Materials Chemistry Frontiers

Sensitive and specific detection of peroxynitrite and in vivo imaging of inflammation by a “simple” AIE bioprobe†

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Sensitive and specific detection of peroxynitrite and in vivo imaging of inflammation by a “simple” AIE bioprobe

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