Issue 99, 2022

Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Abstract

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.

Graphical abstract: Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Supplementary files

Article information

Article type
Communication
Submitted
31 avq 2022
Accepted
09 noy 2022
First published
21 noy 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 13724-13727

Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

M. Trumpp, A. Oliveras, H. Gonschior, J. Ast, D. J. Hodson, P. Knaus, M. Lehmann, M. Birol and J. Broichhagen, Chem. Commun., 2022, 58, 13724 DOI: 10.1039/D2CC04823J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements