Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Michael Trumpp; Anna Oliveras; Hannes Gonschior; Julia Ast; David J. Hodson; Petra Knaus; Martin Lehmann; Melissa Birol; Johannes Broichhagen

doi:10.1039/D2CC04823J

Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy†

Michael Trumpp,

^ab Anna Oliveras,^c Hannes Gonschior,^a Julia Ast,

^d David J. Hodson,

^de Petra Knaus,

^b Martin Lehmann,^a Melissa Birol*^c and Johannes Broichhagen

*^a

Author affiliations

* Corresponding authors

^a Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany
E-mail: broichhagen@fmp-berlin.de

^b Freie Universität Berlin, Institute of Chemistry and Biochemistry – Biochemistry, Thielallee 63, 14195 Berlin, Germany

^c Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine, Hannoversche Str. 28, 10115 Berlin, Germany
E-mail: Melissa.Birol@mdc-berlin.de

^d Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK

^e Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), NIHR Oxford Biomedical Research Centre, Churchill Hospital, Radcliffe Department of Medicine, University of Oxford, Oxford, UK

Abstract

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.

This article is part of the themed collection: 2022 Emerging Investigators

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DOI: https://doi.org/10.1039/D2CC04823J
Article type: Communication
Submitted: 31 avq 2022
Accepted: 09 noy 2022
First published: 21 noy 2022
This article is Open Access

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Chem. Commun., 2022,58, 13724-13727

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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

M. Trumpp, A. Oliveras, H. Gonschior, J. Ast, D. J. Hodson, P. Knaus, M. Lehmann, M. Birol and J. Broichhagen, Chem. Commun., 2022, 58, 13724 DOI: 10.1039/D2CC04823J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy†

Abstract

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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

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