Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Yueci Wu; Hai-Hao Han; Liu He; Li Li; Yi Zang; Jia Li; Xiao-Peng He; Yaping Ding; Weiguo Cao; Tony D. James

doi:10.1039/D2CC06425A

Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore†

Yueci Wu,

‡^a Hai-Hao Han,

‡^def Liu He,^c Li Li,^b Yi Zang,

^d Jia Li,

*^def Xiao-Peng He,

*^c Yaping Ding,

*^b Weiguo Cao

*^b and Tony D. James

*^ag

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Bath, Bath, UK
E-mail: t.d.james@bath.ac.uk

^b Department of Chemistry, Shanghai University, Shanghai 200444, China
E-mail: wgcao@shu.edu.cn

^c Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China
E-mail: xphe@ecust.edu.cn

^d State Key Laboratory of Drug Research, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
E-mail: jli@simm.ac.cn

^e University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, China

^f Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong 264117, China

^g School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China

Abstract

Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO⁻ rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO⁻ in HepG2 cells and endogenous ONOO⁻ induced by LPS in RAW 267.4 cells was demonstrated.

This article is part of the themed collection: Chemosensors and Molecular Logic

Chemical Communications

Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore†

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Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

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