Reduction in insect attachment ability by biogenic and non-biogenic ZnO nanoparticles

Abstract

Nanomaterials can represent an environmentally safe method to control different insect pests. The present study investigated the effect of biogenic and non-biogenic zinc oxide nanoparticles (ZnO-NPs) on the attachment ability of the Southern green stink bug, Nezara viridula, a major agricultural pest. The experiments were conducted on glass surfaces treated with different concentrations of ZnO-NPs, and the attachment ability of adult males of N. viridula was measured through traction force experiments. The results showed that both biogenic and non-biogenic ZnO-NPs reduced the attachment ability of N. viridula, with a significant decrease in traction force observed at concentrations of 12.5 mg L⁻¹ and above. SEM analyses revealed that biogenic and non-biogenic ZnO-NPs aggregated on the attachment devices of N. viridula, including the pulvilli, the hairy pad and claws, disrupting the attachment mechanism. This study suggests that ZnO-NPs, particularly biogenic ZnO-NPs obtained from plant extract, have potential as a natural and eco-friendly pest control agent to reduce insect attachment and damage to crops.