19F NMR ON/OFF nanoparticles: a universal approach for the specific detection of DNA-binding cancer biomarkers

Devanathan Perumal; Jithu Krishna; Kaloor S. Harikrishnan; Gowtham Raj; Jemshiya Kalathil; Minu Saji; Kavyasree M.; Reji Varghese

doi:10.1039/D3NR01175E

¹⁹F NMR ON/OFF nanoparticles: a universal approach for the specific detection of DNA-binding cancer biomarkers†

Devanathan Perumal,^a Jithu Krishna,^a Kaloor S. Harikrishnan,^a Gowtham Raj,^a Jemshiya Kalathil,^a Minu Saji,^a Kavyasree M.^a and Reji Varghese

*^a

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* Corresponding authors

^a School of Chemistry, Indian Institute of Science Education and Research (IISER) Thiruvananthapuram, Trivandrum 695541, Kerala, India
E-mail: reji@iisertvm.ac.in

Abstract

A supramolecular approach for the design of assembly–disassembly-driven ¹⁹F ON/OFF nanoparticles, triggered by specific molecular recognition, for the detection of DNA binding cancer biomarkers is reported. The key to our design strategy is the characteristic ¹⁹F NMR signal of the probe, which completely vanishes in the aggregated state due to the shortening of T₂ relaxation. However, molecular recognition of DNA by the cancer biomarkers through specific molecular recognition results in the disassembly of the nanoparticles, which causes the restoration of the characteristic ¹⁹F signal of the probe. The universal nature of the approach is demonstrated through the selective detection of various cancer biomarkers including miRNA, ATP, thrombin, and telomerase.

This article is part of the themed collection: Emerging concepts in nucleic acids: structures, functions and applications

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Article information

DOI: https://doi.org/10.1039/D3NR01175E
Article type: Communication
Submitted: 14 mar 2023
Accepted: 19 apr 2023
First published: 19 apr 2023
This article is Open Access

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Nanoscale, 2023,15, 8972-8977

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¹⁹F NMR ON/OFF nanoparticles: a universal approach for the specific detection of DNA-binding cancer biomarkers

D. Perumal, J. Krishna, K. S. Harikrishnan, G. Raj, J. Kalathil, M. Saji, K. M. and R. Varghese, Nanoscale, 2023, 15, 8972 DOI: 10.1039/D3NR01175E

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