Iron Fenton oxidation of 2′-deoxyguanosine in physiological bicarbonate buffer yields products consistent with the reactive oxygen species carbonate radical anion not the hydroxyl radical

Abstract

Product analysis from the iron Fenton oxidation of 2′-deoxyguanosine found reactions in bicarbonate buffer yield 8-oxo-2′-deoxyguanosine and spiroiminodihyantoin consistent with CO₃˙⁻. Reactions in phosphate buffer furnished high yields of sugar oxidation products consistent with HO˙. These observations change the view of DNA oxidation products from the iron-Fenton reaction.