Issue 31, 2022

Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy

Abstract

Water suppression is of paramount importance for many biological and analytical NMR spectroscopy applications. Here, we report the design of a new set of binomial-like radio frequency (RF) pulses that elude water irradiation while exciting or refocusing the remainder of the 1H spectrum. These pulses were generated using a combination of an evolutionary algorithm and artificial intelligence. They display higher sensitivity relative to classical water suppression schemes and tunable water selectivity to avoid suppressing 1H resonances near the water signal. The broad bandwidth excitation obtained with these RF pulses makes them suitable for several NMR applications at high and ultra-high-field magnetic fields.

Graphical abstract: Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
14 apr 2022
Accepted
19 iyl 2022
First published
19 iyl 2022

Phys. Chem. Chem. Phys., 2022,24, 18477-18481

Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy

V. S. Manu, C. Olivieri, K. Pavuluri and G. Veglia, Phys. Chem. Chem. Phys., 2022, 24, 18477 DOI: 10.1039/D2CP01744J

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