Issue 14, 2014
From the journal:

Analytical Methods

HPLC separation of casein components on a diol-bonded silica column with MALDI TOF/TOF MS identification†

Paweł Pomastowski,a   Justyna Walczak,a   Marta Gawin,b   Szymon Bocian,a   Wojciech Piekoszewskibc  and  Bogusław Buszewski*a  

* Corresponding authors

a Department of the Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 7 Gagarin Street, PL 87-100 Torun, Poland
E-mail: bbusz@chem.umk.pl
Fax: +48 56 6114837
Tel: +48 56 6114753

b Laboratory of High Resolution Mass Spectrometry, Regional Laboratory of Physicochemical Analysis and Structural Research, Faculty of Chemistry, Jagiellonian University, Ingardena 3, PL 30-060 Krakow, Poland
E-mail: wojciech.piekoszewski@uj.edu.pl

c Toxicological and Pharmaceutical Analysis Group, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, PL 30-060 Krakow, Poland

Abstract

A comparative study has been made of three home-made bonded silica columns for the separation of the components of bovine milk casein (α-, β- and κ-casein). A gradient elution HPLC procedure is now proposed for separation of the protein components, and the tests conducted have confirmed the superiority of columns based on diol-bonded silica over octadecyl- or amine-bonded silica. A matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer (MALDI-TOF MS) has been utilized as a detector for off-line identification of the components of the casein fractions. The method selected enables effective and rapid separation of the casein components, together with their detailed MS characterization.

Graphical abstract: HPLC separation of casein components on a diol-bonded silica column with MALDI TOF/TOF MS identification

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DOI
https://doi.org/10.1039/C4AY00895B
Article type
Paper
Submitted
14 Apr 2014
Accepted
16 May 2014
First published
13 Jun 2014

Anal. Methods, 2014,6, 5236-5244

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HPLC separation of casein components on a diol-bonded silica column with MALDI TOF/TOF MS identification

P. Pomastowski, J. Walczak, M. Gawin, S. Bocian, W. Piekoszewski and B. Buszewski, Anal. Methods, 2014, 6, 5236 DOI: 10.1039/C4AY00895B

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