Issue 37, 2015
From the journal:

Chemical Communications

Transcription of 4′-thioDNA templates to natural RNA in vitro and in mammalian cells†

Hideto Maruyama,a   Kazuhiro Furukawa,b   Hiroyuki Kamiya,c   Noriaki Minakawa*b  and  Akira Matsuda*a  

* Corresponding authors

a Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan
E-mail: matuda@pharm.hokudai.ac.jp

b Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1-78-1, Tokushima 770-8505, Japan
E-mail: minakawa@tokushima-u.ac.jp

c Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan

Abstract

Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies. A comprehensive analysis of the transcription of 4′-thioDNA templates to natural RNA is herein reported. The modifications of DNA with 2′-deoxy-4′-thionucleosides, so-called 4′-thioDNA, were found to act as templates for transcription with RNA polymerases both in vitro and in mammalian cells.

Graphical abstract: Transcription of 4′-thioDNA templates to natural RNA in vitro and in mammalian cells

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Article information

DOI
https://doi.org/10.1039/C4CC08862J
Article type
Communication
Submitted
06 Nov 2014
Accepted
09 Mar 2015
First published
10 Mar 2015

Chem. Commun., 2015,51, 7887-7890

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Transcription of 4′-thioDNA templates to natural RNA in vitro and in mammalian cells

H. Maruyama, K. Furukawa, H. Kamiya, N. Minakawa and A. Matsuda, Chem. Commun., 2015, 51, 7887 DOI: 10.1039/C4CC08862J

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