HIV microarray for the mapping and characterization of HIV-specific antibody responses

Daniela Gallerano; Eva Wollmann; Christian Lupinek; Thomas Schlederer; Daniel Ebner; Christian Harwanegg; Katarzyna Niespodziana; Klaus Schmetterer; Winfried Pickl; Elisabeth Puchhammer-Stöckl; Elopy Sibanda; Rudolf Valenta

doi:10.1039/C4LC01510J

HIV microarray for the mapping and characterization of HIV-specific antibody responses†

Daniela Gallerano,‡^a Eva Wollmann,‡^a Christian Lupinek,‡^a Thomas Schlederer,‡^b Daniel Ebner,‡^b Christian Harwanegg,‡^b Katarzyna Niespodziana,‡^a Klaus Schmetterer,‡^c Winfried Pickl,‡^d Elisabeth Puchhammer-Stöckl,‡^e Elopy Sibanda ‡^f and Rudolf Valenta ‡*^a

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* Corresponding authors

^a Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Währinger Gürtel 18-20, 3Q, 1090 Vienna, Austria
E-mail: rudolf.valenta@meduniwien.ac.at
Fax: +43 1 40400 51300
Tel: +43 1 40400 51080

^b Phadia Austria GmbH, Part of Thermo Fisher Scientific ImmunoDiagnostics, Vienna, Austria

^c Department of Laboratory Medicine, Medical University of Vienna, Austria

^d Department of Immunology, Medical University of Vienna, Austria

^e Department of Virology, Medical University of Vienna, Austria

^f Asthma, Allergy and Immune Dysfunction Clinic, Parirenyatwa University Teaching Hospital, Harare, Zimbabwe

Abstract

We used the microarray technology to develop chips containing a comprehensive set of proteins and peptides covering the proteome of HIV-1 clade C, which is the HIV-1 subtype that causes the majority of infections worldwide. We demonstrate that the HIV microarray allows simultaneous, sensitive and specific detection of antibody responses for the major immunoglobulin classes (IgG, IgA, IgM, IgE) and subclasses (IgG_1–4) with minute amounts of serum samples towards a large number of HIV antigens and peptides. Furthermore, we show that the HIV chip can be used for the monitoring of antibody responses during the course of the disease and during treatment. The HIV microarray should be useful to study antibody responses to multiple HIV antigens and epitopes in HIV-infected patients to explore pathomechanisms of the disease, for diagnosis and for monitoring of treatment and of vaccine trials.

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HIV microarray for the mapping and characterization of HIV-specific antibody responses†

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HIV microarray for the mapping and characterization of HIV-specific antibody responses

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