Issue 81, 2016
From the journal:

RSC Advances

SPECT imaging of cytochrome c in pressure overload mice hearts

Cong Wang,a   Xiao Li,b   Cheng Shen,a   Leilei Ma,a   Zhen Dong,a   Hong Zhu,a   Peng Wang,a   Junbo Geac  and  Aijun Sun*ac  

* Corresponding authors

a Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Fenglin Road 180, Shanghai 200032, PR China
E-mail: sun.aijun@zs-hospital.sh.cn

b Department of Nuclear Medicine, Changhai Hospital, Second Military Medical University, Changhai Road 168, Shanghai 200433, PR China

c Institute of Biomedical Science, Fudan University, Dongan Road 131, Shanghai 200032, PR China

Abstract

Clinically, pressure overload (PO) occurs in many clinical settings such as hypertension and valvular stenosis especially in the current aging society. During cardiac remodeling induced by PO, mitochondria were injured and cytochrome c was released. However, few studies have targeted cytochrome c for imaging. In this study, Technium-99m labeled cytochrome c antibody was developed to image PO hearts induced by transverse aortic constriction surgery. 99mTc-Anti-cytochrome c was available to image and differentiate cytochrome c in normal mice hearts and PO hearts. The findings on images were consistent with detection by cardiac function and molecular biological assay. Hence, the noninvasive targeted cytochrome c imaging of mice hearts can be realized via 99mTc-Anti-cytochrome c based SPECT.

Graphical abstract: SPECT imaging of cytochrome c in pressure overload mice hearts

About
Cited by
Related
Download options Please wait...

Associated articles

Article information

DOI
https://doi.org/10.1039/C6RA18224K
Article type
Paper
Submitted
18 Jul 2016
Accepted
07 Aug 2016
First published
10 Aug 2016

RSC Adv., 2016,6, 77126-77132

Permissions

Request permissions

SPECT imaging of cytochrome c in pressure overload mice hearts

C. Wang, X. Li, C. Shen, L. Ma, Z. Dong, H. Zhu, P. Wang, J. Ge and A. Sun, RSC Adv., 2016, 6, 77126 DOI: 10.1039/C6RA18224K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements