Issue 5, 2017
From the journal:

RSC Advances

Regulating C4-dicarboxylate transporters for improving fumaric acid production

Ting Zhang, ORCID logo †ac   Ruirui Song,†a   Meng Wang,a   Li Deng,*ab   Lihai Fana  and  Fang Wanga  

* Corresponding authors

a National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing, People's Republic of China
E-mail: dengli@mail.buct.edu.cn

b Amoy—BUCT Industrial Bio-technovation Institute, Amoy, People's Republic of China

c The 302 Hospital of PLA, China

Abstract

Although many efforts have been made to engineer Escherichia coli for fumaric acid production, the fumarate efflux system has not been investigated as an engineering target to improve fumaric acid production. In this work, we cloned and expressed C4-dicarboxylate transporters of different sources in a previously constructed fumaric-acid-producing strain to study their effects on the production of fumaric acid. In addition, each native C4-dicarboxylate transporter was deleted in separate experiments to investigate their individual effects on fumaric acid production. The results showed that the expression of the genes dcuB-Ec and dcuC-Ec can increase the fumaric acid yield by 48.5% and 53.1%, respectively. Fed-batch cultivations in a 5 L bioreactor of strain A-dcuB-Ec produced 9.42 g L−1 of fumaric acid after 50 hours.

Graphical abstract: Regulating C4-dicarboxylate transporters for improving fumaric acid production

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Article information

DOI
https://doi.org/10.1039/C6RA24727J
Article type
Paper
Submitted
04 Oct 2016
Accepted
20 Dec 2016
First published
12 Jan 2017
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2017,7, 2897-2904

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Regulating C4-dicarboxylate transporters for improving fumaric acid production

T. Zhang, R. Song, M. Wang, L. Deng, L. Fan and F. Wang, RSC Adv., 2017, 7, 2897 DOI: 10.1039/C6RA24727J

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