Issue 21, 2020
From the journal:

Analyst

A bifunctional DNA probe for sensing pH and microRNA using a nanopore†

Yun Zhang,‡a   Peng Song,‡b   Bingyuan Guo,*a   Wenying Hao,a   Lei Liu ORCID logo c  and  Hai-Chen Wu ORCID logo *ad  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
E-mail: haichenwu@iccas.ac.cn
Tel: +8610-82614948

b Department of Geriatric Oncology, General Hospital of the Chinese People's Liberation Army, Beijing 100853, China

c Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China

d University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

We have developed a bifunctional probe based on triplex molecular beacons for the measurement of environmental pH and quantification of microRNA-10b using a nanopore. The probe responds sharply to solution pH changes in the range of 6.0–7.5. The limit of detection for microRNA-10b is as low as 5.0 pM.

Graphical abstract: A bifunctional DNA probe for sensing pH and microRNA using a nanopore

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Article information

DOI
https://doi.org/10.1039/D0AN01208D
Article type
Paper
Submitted
16 Jun 2020
Accepted
09 Aug 2020
First published
10 Aug 2020

Analyst, 2020,145, 7025-7029

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A bifunctional DNA probe for sensing pH and microRNA using a nanopore

Y. Zhang, P. Song, B. Guo, W. Hao, L. Liu and H. Wu, Analyst, 2020, 145, 7025 DOI: 10.1039/D0AN01208D

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