Issue 24, 2024

Re-pairing DNA: binding of a ruthenium phi complex to a double mismatch

Abstract

We report a crystal structure at atomic resolution (0.9 Å) of a ruthenium complex bound to a consecutive DNA double mismatch, which results in a TA basepair with flipped out thymine, together with the formation of an adenine bulge. The structure shows a form of metalloinsertion interaction of the Λ-[Ru(phen)2phi]2+ (phi = 9,10-phenanthrenediimine) complex at the bulge site. The metal complex interacts with the DNA via the major groove, where specific interactions between the adenines of the DNA and the phen ligands of the complex are formed. One Δ-[Ru(phen)2phi]2+ complex interacts via the minor groove, which shows sandwiching of its phi ligand between the phi ligands of the other two ruthenium complexes, and no interaction of its phen ligands with DNA. To our knowledge, this binding model represents a new form of metalloinsertion in showing major rather than minor groove insertion.

Graphical abstract: Re-pairing DNA: binding of a ruthenium phi complex to a double mismatch

Associated articles

Supplementary files

Article information

Article type
Edge Article
Submitted
01 Mar 2024
Accepted
11 May 2024
First published
16 May 2024
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2024,15, 9096-9103

Re-pairing DNA: binding of a ruthenium phi complex to a double mismatch

T. D. Prieto Otoya, K. T. McQuaid, N. G. Paterson, D. J. Cardin, A. Kellett and C. J. Cardin, Chem. Sci., 2024, 15, 9096 DOI: 10.1039/D4SC01448K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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