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This communication reports on a fluorescent probe (PPI-P) for imaging active peptidyl-prolyl cis/trans isomerases in live cells. PPI-P is capable of responding to both recombinant and cellular PPIases fluorogenically, and has been shown to specifically image active PPIases in live cells.

Graphical abstract: A fluorescent peptidyl substrate for visualizing peptidyl-prolyl cis/trans isomerase activity in live cells

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