Biosynthesis of the meroterpenoid metabolite, andibenin B: incorporation of sodium [1-13C, 18O2]acetate and 18O2
Abstract
Incorporation of sodium [1-13C, 18O2]acetate and 18O2 gas into andibenin B (1) by cultures of Aspergillus variecolor and observation of 18O isotope-induced shifts in the 13C n.m.r. spectra of the enriched metabolites establish the origins of all the oxygen atoms and provide mechanistic information on the biosynthetic pathway.