Characterisation of a hydroxymandelate oxidase involved in the biosynthesis of two unusual amino acids occurring in the vancomycin group of antibioticsElectronic supplementary information (ESI) available: SDS-PAGE analysis of HmO and molecular weight analysis of HmO protein by ESI-MS and GC/CI-MS of the enzymatic conversion of 3,5DHMA with HmO. See http://www.rsc.org/suppdata/cc/b1/b103548g/

Abstract

ORF22 from the chloroeremomycin gene cluster has been cloned, expressed and characterised as a hydroxymandelate oxidase (HmO) that is involved in the formation of both (S)-4-hydroxyphenylglycine and (S)-3,5-dihydroxyphenylglycine.