Issue 11, 2002

Determination of catalase activity in samples treated with [ZnCl2(isopropylamine)2]: a novel zinc complex that slows down the decay in activity of catalase extracts

Abstract

Reaction of ZnCl2 with an excess of isopropylamine ligand in water gives [ZnCl2(isopropylamine)2]. This novel zinc complex has been characterized by elemental analysis and 1H-NMR. Interestingly, we have found that [ZnCl2(isopropylamine)2] markedly retards the decay in activity of catalase extracts. In fact, catalase extracts (0.075 mg of protein ml−1) may retain more than 80% of their initial enzymatic activity within the first 10 hours of incubation with 0.10 μM [ZnCl2(isopropylamine)2] while the activity of control extracts decreases to less than 50% of the initial value. Moreover, after 24 hours of treatment with [ZnCl2(isopropylamine)2] under the above-mentioned conditions, 70% of the initial enzymatic activity is still retained by catalase extracts while the activity of control untreated extracts drops to 17% of the initial value. On the other hand, our results show that after incubation of catalase extracts with [ZnCl2(isopropylamine)2] the supernatants obtained by centrifugation of the extracts contain a higher amount of active catalase than the supernatants of control untreated catalase extracts. Moreover, [ZnCl2(isopropylamine)2] induces precipitation of a large amount of contaminant proteins present in the catalase extracts. Altogether, these data indicate that treatment of catalase extracts with [ZnCl2(isopropylamine)2] both increases the pureness of catalase solutions and slows down the decay in catalase activity. We believe that [ZnCl2(isopropylamine)2] may be useful as a stabilizing agent for enzyme activity assays with crude catalase extracts.

Graphical abstract: Determination of catalase activity in samples treated with [ZnCl2(isopropylamine)2]: a novel zinc complex that slows down the decay in activity of catalase extracts

Article information

Article type
Paper
Submitted
31 Jan 2002
Accepted
14 Mar 2002
First published
30 Apr 2002

J. Chem. Soc., Dalton Trans., 2002, 2283-2288

Determination of catalase activity in samples treated with [ZnCl2(isopropylamine)2]: a novel zinc complex that slows down the decay in activity of catalase extracts

J. García-Cañadas, J. M. Pérez, A. G. Quiroga, M. A. Fuertes, C. Alonso and C. Navarro-Ranninger, J. Chem. Soc., Dalton Trans., 2002, 2283 DOI: 10.1039/B201151B

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