Issue 12, 2005

Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique

Abstract

We already found that β-1,3-glucan polysaccharides form polymeric complexes with certain polynucleotides, but the parallel vs. anti-parallel orientation in those complexes had remained unsolved. In this paper, this controversial problem has been discussed for curdlan/oligo(dA) complexes utilizing two different energy transfer techniques. The first system consists of a combination of fluorescein-labeled curdlan and 3′-(or 5′-)tetramethyl-rhodamine (TAMRA)-labeled oligo(dA). The second system utilizes gold nanoparticles: that is, two curdlan chains were linked by a disulfide bond and after complexation with oligo(dA), the complex was immobilized on gold nanoparticles. In this system, TAMRA was attached to the 3′ (or 5′) end of oligo(dA) and the gold particle acted as a fluorescence quencher (energy acceptor). These experiments have led us to conclude that in the curdlan/oligo(dA) complex, parallel orientation is more favourable than anti-parallel orientation. These findings have enabled us to envision a clearer image for the complexation mode between β-1,3-glucan polysaccharides and polynucleotides.

Graphical abstract: Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique

Article information

Article type
Paper
Submitted
07 Jan 2005
Accepted
25 Apr 2005
First published
10 May 2005

Org. Biomol. Chem., 2005,3, 2255-2261

Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique

M. Numata, K. Koumoto, M. Mizu, K. Sakurai and S. Shinkai, Org. Biomol. Chem., 2005, 3, 2255 DOI: 10.1039/B500156K

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