Issue 33, 2006

Supported phospholipid bilayer formation on hydrophilicity-controlled silicon dioxide surfaces

Abstract

We investigated the influence of surface hydroxyl groups (–OHs) on the supported planar phospholipid bilayer (SPB) formation and characteristics. We prepared SiO2 surfaces with different hydrophilicity degree by annealing the SiO2 layer on Si(100) formed by wet chemical treatments. The hydrophilicity reduced with irreversible thermal desorption of –OHs. We formed SPB of dimyristoylphosphatidylcholine on the SiO2 surfaces by incubation at a 100-nm-filtered vesicle suspension. The formation rate was faster on less hydrophilic surfaces. We proposed that a stable hydrogen-bonded water layer on the SiO2 surface worked as a barrier to prevent vesicle adhesion on the surface. Theoretical calculation indicates that water molecules on vicinal surface –OHs take a stable surface-unique geometry, which disappears on an isolated –OH. The surface –OH density, however, affected little the fluidity of once formed SPBs, which was measured by the fluorescence recovery after the photobleaching method. We also describe the area-selective SPB deposition using surface patterning by the focused ion beam.

Graphical abstract: Supported phospholipid bilayer formation on hydrophilicity-controlled silicon dioxide surfaces

Article information

Article type
Paper
Submitted
28 Apr 2006
Accepted
06 Jul 2006
First published
21 Jul 2006

Phys. Chem. Chem. Phys., 2006,8, 3885-3894

Supported phospholipid bilayer formation on hydrophilicity-controlled silicon dioxide surfaces

R. Tero, H. Watanabe and T. Urisu, Phys. Chem. Chem. Phys., 2006, 8, 3885 DOI: 10.1039/B606052H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements