Issue 20, 2008
From the journal:

Organic & Biomolecular Chemistry

Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA†

Hana Cahová,a   Radek Pohl,a   Lucie Bednárová,a   Kateřina Nováková,a   Josef Cvačkaa  and  Michal Hocek*a  

* Corresponding authors

a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead & IOCB Research Center, Flemingovo nam. 2, Prague 6, Czech Republic
E-mail: hocek@uochb.cas.cz
Fax: +420 220183559
Tel: +420 220183324

Abstract

dATP derivatives bearing Br, Me or Ph groups in position 8 were prepared and tested as substrates for DNA polymerases to show that 8-Br-dATP and 8-Me-dATP were efficiently incorporated, while 8-Ph-dATP was a poor substrate due to its bulky Ph group.

Graphical abstract: Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA

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Article information

DOI
https://doi.org/10.1039/B811935J
Article type
Communication
Submitted
11 Jul 2008
Accepted
29 Aug 2008
First published
17 Sep 2008

Org. Biomol. Chem., 2008,6, 3657-3660

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Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA

H. Cahová, R. Pohl, L. Bednárová, K. Nováková, J. Cvačka and M. Hocek, Org. Biomol. Chem., 2008, 6, 3657 DOI: 10.1039/B811935J

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