Highly selective detection of Cu2+ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu2+ ion viasurface plasmon resonance spectroscopy

Suseung Lee; Inhee Choi; Surin Hong; Young In Yang; Jeongjin Lee; Taewook Kang; Jongheop Yi

doi:10.1039/B910666A

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Highly selective detection of Cu²⁺ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu²⁺ ion viasurface plasmon resonance spectroscopy†

Suseung Lee,^a Inhee Choi,^a Surin Hong,^a Young In Yang,^a Jeongjin Lee,^a Taewook Kang*^b and Jongheop Yi*^a

Author affiliations

* Corresponding authors

^a School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 151-744, Korea
E-mail: jyi@snu.ac.kr
Fax: +82-2-885-6670
Tel: +82-2-880-7438

^b Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Korea
E-mail: twkang@sogang.ac.kr
Fax: +82-2-885-6670
Tel: +82-2-880-7438

Abstract

A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and in situ detect Cu²⁺ without any interference that other spectroscopic methods may have.

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Supplementary files

Selectivity test in mixed metal ion solution PDF (98K)

Article information

DOI: https://doi.org/10.1039/B910666A
Article type: Communication
Submitted: 01 Jun 2009
Accepted: 22 Aug 2009
First published: 11 Sep 2009

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Chem. Commun., 2009, 6171-6173

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Highly selective detection of Cu²⁺ utilizing specific binding between Cu-demetallated superoxide dismutase 1 and the Cu²⁺ ion viasurface plasmon resonance spectroscopy

S. Lee, I. Choi, S. Hong, Y. In Yang, J. Lee, T. Kang and J. Yi, Chem. Commun., 2009, 6171 DOI: 10.1039/B910666A

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Chemical Communications