Issue 12, 2010
From the journal:

Analyst

Infrared microspectroscopy of live cells in aqueous media†

Ellen J. (Swain) Marcsisin,*a   Christina M. Uttero,a   Miloš Miljkovića  and  Max Diema  

* Corresponding authors

a Laboratory for Spectral Diagnosis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA

Abstract

Fourier Transform Infrared (FTIR) spectroscopic measurements of individual, live HeLa cells in culture and buffer media are presented. Spectral data were acquired using a newly designed live cell chamber developed in the authors' laboratory. Data were processed using MATLAB-based routines that correct for the overcompensation of water encountered during live cell measurements in aqueous samples. Data presented are from live cells monitored over an extended period of time as well as a comparison of live cells exposed to perturbing conditions.

Graphical abstract: Infrared microspectroscopy of live cells in aqueous media

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Article information

DOI
https://doi.org/10.1039/C0AN00548G
Article type
Paper
Submitted
19 Jul 2010
Accepted
11 Oct 2010
First published
28 Oct 2010

Analyst, 2010,135, 3227-3232

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Infrared microspectroscopy of live cells in aqueous media

E. J. (Swain) Marcsisin, C. M. Uttero, M. Miljković and M. Diem, Analyst, 2010, 135, 3227 DOI: 10.1039/C0AN00548G

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