Tailored adsorption of His6-tagged protein onto nickel(II)–cyclam grafted mesoporous silica

Darragh A. Gaffney; Sarah O’Neill; Mary C. O’Loughlin; Ulf Hanefeld; Jakki C. Cooney; Edmond Magner

doi:10.1039/B915169A

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Tailored adsorption of His₆-tagged protein onto nickel(ii)–cyclam grafted mesoporous silica†

Darragh A. Gaffney,^a Sarah O’Neill,^a Mary C. O’Loughlin,^b Ulf Hanefeld,^c Jakki C. Cooney*^b and Edmond Magner*^a

Author affiliations

* Corresponding authors

^a Materials and Surface Science Institute, SFI-SRC in Solar Energy Conversion, Department of Chemical and Environmental Science, University of Limerick, Limerick, Ireland
E-mail: Edmond.Magner@ul.ie
Tel: +353-61-202629

^b Materials and Surface Science Institute, Department of Life Sciences, University of Limerick, Limerick, Ireland
E-mail: Jakki.Cooney@ul.ie
Tel: +353-61-202880

^c Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft, The Netherlands
E-mail: U.Hanefeld@tudelft.nl
Tel: +31-15-2789304

Abstract

The specific immobilisation of a histidine-tagged protein, Spi, onto mono-anchored nickel(II)–cyclam functionalised SBA-15 is reported.

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Supplementary files

Synthesis of SBA-15–Ni–cyclam, synthesis of His6-Spi and Spi, enzyme adsorption procedure, leaching experiments, characterisation data; nitrogen adsorption analysis, low-angle XRD, XPS PDF (264K)

Article information

DOI: https://doi.org/10.1039/B915169A
Article type: Communication
Submitted: 29 Jul 2009
Accepted: 09 Dec 2009
First published: 23 Dec 2009

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Chem. Commun., 2010,46, 1124-1126

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Tailored adsorption of His₆-tagged protein onto nickel(II)–cyclam grafted mesoporous silica

D. A. Gaffney, S. O’Neill, M. C. O’Loughlin, U. Hanefeld, J. C. Cooney and E. Magner, Chem. Commun., 2010, 46, 1124 DOI: 10.1039/B915169A

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