Issue 1, 2010
From the journal:

Chemical Communications

Bioluminescent nanosensors for protease detection based upon gold nanoparticle–luciferase conjugates†

Young-Pil Kim,a   Weston L. Daniel,b   Zuyong Xia,a   Hexin Xie,a   Chad A. Mirkinb  and  Jianghong Rao*a  

* Corresponding authors

a Molecular Imaging Program at Stanford (MIPS), Departments of Radiology and Chemistry, Stanford University, Stanford, USA
E-mail: jrao@stanford.edu
Fax: +1 650 736 7925
Tel: +1 650 736 8563

b Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, USA
E-mail: chadnano@northwestern.edu
Fax: +1 847 467 5123

Abstract

This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.

Graphical abstract: Bioluminescent nanosensors for protease detection based upon gold nanoparticle–luciferase conjugates

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Article information

DOI
https://doi.org/10.1039/B915612G
Article type
Communication
Submitted
30 Jul 2009
Accepted
28 Sep 2009
First published
12 Oct 2009

Chem. Commun., 2010,46, 76-78

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Bioluminescent nanosensors for protease detection based upon gold nanoparticle–luciferase conjugates

Y. Kim, W. L. Daniel, Z. Xia, H. Xie, C. A. Mirkin and J. Rao, Chem. Commun., 2010, 46, 76 DOI: 10.1039/B915612G

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