Issue 13, 2010
From the journal:

Chemical Communications

Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples†

P. L. Urban,a   A. Amantonico,a   S. R. Fagerer,a   P. Gehrigb  and  R. Zenobi*a  

* Corresponding authors

a Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland
E-mail: zenobi@org.chem.ethz.ch
Fax: +41 44 6321292
Tel: +41 44 6324377

b Functional Genomics Center Zurich, UZH/ETH Zurich, CH-8057 Zurich, Switzerland

Abstract

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

Graphical abstract: Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

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Article information

DOI
https://doi.org/10.1039/B925433A
Article type
Communication
Submitted
02 Dec 2009
Accepted
08 Feb 2010
First published
25 Feb 2010

Chem. Commun., 2010,46, 2212-2214

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Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

P. L. Urban, A. Amantonico, S. R. Fagerer, P. Gehrig and R. Zenobi, Chem. Commun., 2010, 46, 2212 DOI: 10.1039/B925433A

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