Issue 38, 2010
From the journal:

Chemical Communications

Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine†

Renliang Yang,‡a   Kalyan Kumar Pasunooti,‡b   Fupeng Li,a   Xue-Wei Liu*b  and  Chuan-Fa Liu*a  

* Corresponding authors

a Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551
E-mail: cfliu@ntu.edu.sg
Fax: (+65) 6791-3856

b Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
E-mail: xuewei@ntu.edu.sg
Fax: (+65) 6791-3856

Abstract

The dual native chemical ligation at lysine strategy was revised by replacing the acid-labile Cbz protecting group with photolabile NVOC at the 4-mercaptolysine side chain. The optimized strategy was subsequently applied to the synthesis of K48-linked diubiquitin.

Graphical abstract: Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine

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Article information

DOI
https://doi.org/10.1039/C0CC01382J
Article type
Communication
Submitted
13 May 2010
Accepted
05 Aug 2010
First published
25 Aug 2010

Chem. Commun., 2010,46, 7199-7201

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Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine

R. Yang, K. K. Pasunooti, F. Li, X. Liu and C. Liu, Chem. Commun., 2010, 46, 7199 DOI: 10.1039/C0CC01382J

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