Issue 39, 2010

Diazirine-containing RNA photocrosslinking probes for the study of siRNA–protein interactions

Abstract

We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3′-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.

Graphical abstract: Diazirine-containing RNA photocrosslinking probes for the study of siRNA–protein interactions

Supplementary files

Article information

Article type
Communication
Submitted
09 Jul 2010
Accepted
16 Aug 2010
First published
06 Sep 2010

Chem. Commun., 2010,46, 7367-7369

Diazirine-containing RNA photocrosslinking probes for the study of siRNAprotein interactions

S. Kuboe, M. Yoda, A. Ogata, Y. Kitade, Y. Tomari and Y. Ueno, Chem. Commun., 2010, 46, 7367 DOI: 10.1039/C0CC02450C

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