Issue 47, 2010

Nanoparticle based enhancement of electrochemical DNA hybridization signal using nanoporous electrodes

Abstract

A novel nanoparticle-based enhanced methodology for the detection of ssDNA using nanoporous alumina filter membranes, containing pores of 200 nm in diameter, is reported. The blockage of the pores due to the hybridization is detected by measuring the decrease in the differential pulse voltammetric response of the [Fe(CN)6]4−/3− redox indicator and using screen-printed carbon electrodes as transducing platform. Furthermore, 20 nm gold nanoparticle (AuNPs) tags are used in order to increase the sensitivity of the assay. The enhancement mechanism of DNA detection is due to an additional blocking effect induced by hybridization reaction by bringing AuNPs inside the pores. The developed methodology can be extended to other biosensing systems with interest not only for DNA but also for proteins and cells. The developed nanochannel/nanoparticle biosensing system would have enormous potential in future miniaturized designs adapted to mass production technologies such as screen-printing technology.

Graphical abstract: Nanoparticle based enhancement of electrochemical DNA hybridization signal using nanoporous electrodes

Supplementary files

Additions and corrections

Article information

Article type
Communication
Submitted
20 Jul 2010
Accepted
24 Sep 2010
First published
18 Oct 2010

Chem. Commun., 2010,46, 9007-9009

Nanoparticle based enhancement of electrochemical DNA hybridization signal using nanoporous electrodes

A. de la Escosura-Muñiz and A. Mekoçi, Chem. Commun., 2010, 46, 9007 DOI: 10.1039/C0CC02683B

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