Issue 43, 2010
From the journal:

Chemical Communications

Identification of epigenetic DNA modifications with a protein nanopore†

Emma V. B. Wallace,a   David Stoddart,a   Andrew J. Heron,a   Ellina Mikhailova,a   Giovanni Maglia,a   Timothy J. Donohoea  and  Hagan Bayley*a  

* Corresponding authors

a Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, UK
E-mail: hagan.bayley@chem.ox.ac.uk
Fax: +44 (0)1865 275708
Tel: +44 (0)1865 285101

Abstract

Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.

Graphical abstract: Identification of epigenetic DNA modifications with a protein nanopore

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Article information

DOI
https://doi.org/10.1039/C0CC02864A
Article type
Communication
Submitted
28 Jul 2010
Accepted
24 Sep 2010
First published
06 Oct 2010

Chem. Commun., 2010,46, 8195-8197

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Identification of epigenetic DNA modifications with a protein nanopore

E. V. B. Wallace, D. Stoddart, A. J. Heron, E. Mikhailova, G. Maglia, T. J. Donohoe and H. Bayley, Chem. Commun., 2010, 46, 8195 DOI: 10.1039/C0CC02864A

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