DNA strand exchange catalyzed by molecular crowding in PEG solutions

Bobo Feng; Karolin Frykholm; Bengt Nordén; Fredrik Westerlund

doi:10.1039/C0CC03117H

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DNA strand exchange catalyzed by molecular crowding in PEG solutions†

Bobo Feng,^a Karolin Frykholm,^a Bengt Nordén^a and Fredrik Westerlund*^ab

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* Corresponding authors

^a Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
E-mail: fredrik.westerlund@chalmers.se
Fax: +46 31 772 3858
Tel: +46 31 772 3042

^b Department of Physics, University of Gothenburg, Gothenburg, Sweden

Abstract

DNA strand exchange is catalyzed by molecular crowding and hydrophobic interactions in concentrated aqueous solutions of polyethylene glycol, a discovery of relevance for understanding the function of recombination enzymes and with potential applications to DNA nanotechnology.

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Supplementary files

CD spectra of unlabeled D F/ D T in phosphate buffer and 50% PEG at different temperatures, and melting curves of unlabeled D F/ D T at different PEG concentrations PDF (383K)

Article information

DOI: https://doi.org/10.1039/C0CC03117H
Article type: Communication
Submitted: 08 Aug 2010
Accepted: 10 Sep 2010
First published: 29 Sep 2010

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Chem. Commun., 2010,46, 8231-8233

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DNA strand exchange catalyzed by molecular crowding in PEG solutions

B. Feng, K. Frykholm, B. Nordén and F. Westerlund, Chem. Commun., 2010, 46, 8231 DOI: 10.1039/C0CC03117H

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Chemical Communications

DNA strand exchange catalyzed by molecular crowding in PEG solutions†

Abstract

Supplementary files

Article information

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DNA strand exchange catalyzed by molecular crowding in PEG solutions

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