Issue 47, 2010
From the journal:

Chemical Communications

Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring†

M. Carmen Galan,*a   Anh Tuan Trana  and  Claire Bernarda  

* Corresponding authors

a School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK
E-mail: M.C.Galan@bristol.ac.uk
Fax: +44 (0)117 929 8611
Tel: +44 (0)117 928 7654

Abstract

A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.

Graphical abstract: Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

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Article information

DOI
https://doi.org/10.1039/C0CC04224B
Article type
Communication
Submitted
04 Oct 2010
Accepted
21 Sep 2010
First published
25 Oct 2010

Chem. Commun., 2010,46, 8968-8970

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Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring

M. C. Galan, A. T. Tran and C. Bernard, Chem. Commun., 2010, 46, 8968 DOI: 10.1039/C0CC04224B

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