Issue 12, 2010
From the journal:

Nanoscale

Functionalized gold nanoparticles: a detailed in vivo multimodal microscopic brain distribution study†

Fernanda Sousa,‡a   Subhra Mandal,b   Chiara Garrovo,a   Alberto Astolfo,c   Alois Bonifacio,d   Diane Latawiec,§e   Ralf Hendrik Menk,f   Fulvia Arfelli,cg   Sabine Huewel,h   Giuseppe Legname,e   Hans-Joachim Gallah  and  Silke Krol‡*a  

* Corresponding authors

a NanoBioMed Lab @ LANADA (Laboratory for Nanodiagnostics, Drug Delivery and Analysis), CBM - Cluster in Biomedicine S.c.r.l., Basovizza, AREA Science Park, Trieste, Italy
E-mail: silke.krol@istituto-besta.it

b SISSA - Scuola Internazionale Superiore di Studi Avanzati -International School of Advanced Studies, Trieste, Italy

c Department of Physics, University of Trieste, Trieste, Italy

d Centre of Excellence for Nanostructured Materials, University of Trieste, Trieste, Italy

e Laboratory of Prion Biology, Neurobiology Sector, Scuola Internazionale Superiore di Studi Avanzati - International School of Advanced Studies, AREA Science Park, Trieste, Italy

f Sincrotrone Trieste S.C.p.A. di Interesse Nazionale, AREA Science Park, Trieste, Italy

g INFN-Istituto Nationale di Fisica Nucleare, Trieste, Italy

h Institute for Biochemistry, Westf, Wilhelms-University, Muenster, Germany

Abstract

In the present study, the in vivo distribution of polyelectrolyte multilayer coated gold nanoparticles is shown, starting from the living animal down to cellular level. The coating was designed with functional moieties to serve as a potential nano drug for prion disease. With near infrared time-domain imaging we followed the biodistribution in mice up to 7 days after intravenous injection of the nanoparticles. The peak concentration in the head of mice was detected between 19 and 24 h. The precise particle distribution in the brain was studied ex vivo by X-ray microtomography, confocal laser and fluorescence microscopy. We found that the particles mainly accumulate in the hippocampus, thalamus, hypothalamus, and the cerebral cortex.

Graphical abstract: Functionalized gold nanoparticles: a detailed in vivo multimodal microscopic brain distribution study

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Article information

DOI
https://doi.org/10.1039/C0NR00345J
Article type
Paper
Submitted
27 May 2010
Accepted
10 Aug 2010
First published
15 Oct 2010

Nanoscale, 2010,2, 2826-2834

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Functionalized gold nanoparticles: a detailed in vivo multimodal microscopic brain distribution study

F. Sousa, S. Mandal, C. Garrovo, A. Astolfo, A. Bonifacio, D. Latawiec, R. H. Menk, F. Arfelli, S. Huewel, G. Legname, H. Galla and S. Krol, Nanoscale, 2010, 2, 2826 DOI: 10.1039/C0NR00345J

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