Issue 15, 2010
From the journal:

Organic & Biomolecular Chemistry

Fura-2FF-based calciumindicator for proteinlabeling†

Agostina A. Ruggiu,a   Michael Bannwartha  and  Kai Johnsson*a  

* Corresponding authors

a Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
E-mail: kai.johnsson@epfl.ch
Fax: +41 (0)21 693 93 55
Tel: +41 (0)21 693 93 56

Abstract

We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca2+ indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca2+ sensing ability after coupling to protein.

Graphical abstract: Fura-2FF-based calcium indicator for protein labeling

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Article information

DOI
https://doi.org/10.1039/C000158A
Article type
Communication
Submitted
12 Jan 2010
Accepted
04 Jun 2010
First published
16 Jun 2010

Org. Biomol. Chem., 2010,8, 3398-3401

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Fura-2FF-based calcium indicator for protein labeling

A. A. Ruggiu, M. Bannwarth and K. Johnsson, Org. Biomol. Chem., 2010, 8, 3398 DOI: 10.1039/C000158A

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