Issue 22, 2011

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Abstract

A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

Graphical abstract: High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Supplementary files

Article information

Article type
Communication
Submitted
01 Aug 2011
Accepted
14 Sep 2011
First published
29 Sep 2011

Analyst, 2011,136, 4670-4673

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

A. Knežević, J. Bones, S. K. Kračun, O. Gornik, P. M. Rudd and G. Lauc, Analyst, 2011, 136, 4670 DOI: 10.1039/C1AN15684E

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