Issue 12, 2011

Intracellular and extracellular sampling to monitor the neurotransmission process using a chromatographic method

Abstract

The neurotransmission process is essential for cellular communication and in a variety of disorders the mechanism is altered. At present, biological tissue sampling approaches utilise single sampling techniques where either neurochemicals present inside the cell or released from the cell are monitored, yet the neurotransmission mechanism involves both processes. We have utilised a sampling approach that allows for monitoring intracellular and extracellular neurochemicals, using image analysis of the tissue area to normalise neurochemicals levels for both methods. Both ratios and neurochemical levels were presented to explain alterations in neurotransmission mechanism between proximal and distal colon. The new parameters obtained from intracellular and extracellular sampling are more suited to explain neurotransmitter release and clearance compared to conventionally used parameters from single sampling studies.

Graphical abstract: Intracellular and extracellular sampling to monitor the neurotransmission process using a chromatographic method

Supplementary files

Article information

Article type
Paper
Submitted
20 Aug 2011
Accepted
26 Sep 2011
First published
19 Oct 2011

Anal. Methods, 2011,3, 2770-2776

Intracellular and extracellular sampling to monitor the neurotransmission process using a chromatographic method

L. Parmar, L. D. Morgan and B. A. Patel, Anal. Methods, 2011, 3, 2770 DOI: 10.1039/C1AY05520H

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