Issue 22, 2011

Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

Abstract

Templated DNA strand ligation by the ring-strain promoted alkyne–azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.

Graphical abstract: Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

Supplementary files

Article information

Article type
Communication
Submitted
08 Feb 2011
Accepted
12 Apr 2011
First published
06 May 2011

Chem. Commun., 2011,47, 6257-6259

Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

M. Shelbourne, X. Chen, T. Brown and A. H. El-Sagheer, Chem. Commun., 2011, 47, 6257 DOI: 10.1039/C1CC10743G

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