Issue 32, 2011
From the journal:

Chemical Communications

An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification†

Zhenyu Lin,*a   Weiqiang Yang,a   Guiyun Zhang,a   Qida Liu,b   Bin Qiu,a   Zongwei Caic  and  Guonan Chen*a  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, China
E-mail: zylin@fzu.edu.cn, gnchen@fzu.edu.cn
Fax: (+86)-591-22866135

b MOE Key Laboratory for Strength and Vibration, Xi'an Jiaotong University, China

c Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China

Abstract

A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

Graphical abstract: An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

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Article information

DOI
https://doi.org/10.1039/C1CC13146J
Article type
Communication
Submitted
28 May 2011
Accepted
27 Jun 2011
First published
13 Jul 2011

Chem. Commun., 2011,47, 9069-9071

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An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Z. Lin, W. Yang, G. Zhang, Q. Liu, B. Qiu, Z. Cai and G. Chen, Chem. Commun., 2011, 47, 9069 DOI: 10.1039/C1CC13146J

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