13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water

Shinya Hanashima; Koichi Kato; Yoshiki Yamaguchi

doi:10.1039/C1CC13310A

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¹³C-NMR quantification of proton exchange at LewisX hydroxyl groups in water†

Shinya Hanashima,^a Koichi Kato^bc and Yoshiki Yamaguchi*^a

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* Corresponding authors

^a Structural Glycobiology Team, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, 351-0198 Saitama, Japan
E-mail: yyoshiki@riken.jp
Fax: +81 48-467-9620
Tel: +81 48-467-9619

^b Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan

^c Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan

Abstract

NMR-based analysis of glycans by directly observing hydroxyl protons has been difficult because of their inherently fast exchange with water. We observed hydroxyl proton exchanges in a LewisX–LewisX interaction by using deuterium isotope shifts on ¹³C-NMR. This strategy is suitable for analyzing weak interactions by identifying involved protons.

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Article information

DOI: https://doi.org/10.1039/C1CC13310A
Article type: Communication
Submitted: 03 Jun 2011
Accepted: 19 Aug 2011
First published: 05 Sep 2011

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Chem. Commun., 2011,47, 10800-10802

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¹³C-NMR quantification of proton exchange at LewisX hydroxyl groups in water

S. Hanashima, K. Kato and Y. Yamaguchi, Chem. Commun., 2011, 47, 10800 DOI: 10.1039/C1CC13310A

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Chemical Communications