A gram scale synthesis of a multi-13C-labelled anthocyanin, [6,8,10,3′,5′-13C5]cyanidin-3-glucoside, for use in oral tracer studies in humans

Abstract

The major dietary anthocyanin, cyanidin-3-glucoside, was prepared on a 4 g scale from three units of diethyl [2-¹³C]malonate and one unit of [1,3-¹³C₂]acetone, such that five isotope locations were distributed throughout the molecule to provide a penta-¹³C₅-labelled anthocyanin, [6,8,10,3′,5′-¹³C₅]cyanidin-3-glucoside chloride, for use in human stable-isotope tracer studies.