A kinetic study of the interaction of DNA with gold nanoparticles: mechanistic aspects of the interaction

Abstract

A kinetic study of the interaction of gold nanoparticles capped with N-(2-mercaptopropionyl)glycine with double stranded DNA was carried out in water and in salt (NaCl) solutions. The kinetic curves are biexponential and reveal the presence of three kinetic steps. The dependence of the reciprocal fast and slow relaxation time, on the DNA concentration, is a curve and tends to a plateau at high DNA concentrations. The simplest mechanism consistent with the kinetic results involves a simple three-step series mechanism reaction scheme. The first step corresponds to a very fast step that is related to a diffusion controlled formation of an external precursor complex (DNA, AuNPs); the second step involves the formation of a (DNA/AuNPs)_I complex, as a result of the binding affinity between hydrophilic groups of the tiopronin and the DNA grooves. Finally, the third step has been interpreted as a consequence of a conformational change of the (DNA/AuNPs)_I complex formed in the second step, to a more compacted form (DNA/AuNPs)_II. The values of the rate constants of each step decrease as NaCl concentration increases. The results have been discussed in terms of solvation of the species and changes in the viscosity of the solution.