Issue 48, 2011
From the journal:

Physical Chemistry Chemical Physics

Scanning near-field IR microscopy of proteins in lipid bilayers

Fouad Ballout,a   Henning Krassen,bc   Ilona Kopf,ad   Kenichi Ataka,be   Erik Bründermann,a   Joachim Heberlebe  and  Martina Havenith*a  

* Corresponding authors

a Department of Physical Chemistry II, Ruhr-University Bochum, 44801 Bochum, Germany
E-mail: martina.havenith@rub.de

b Department of Chemistry, Biophysical Chemistry, Bielefeld University, 33615 Bielefeld, Germany

c Department of Photochemistry and Molecular Science, Uppsala University, 75120 Uppsala, Sweden

d CRANN, Trinity College Dublin, College Green, Dublin 2, Ireland

e Department of Physics, Experimental Molecular Biophysics, Freie Universität Berlin, 14195 Berlin, Germany

Abstract

We use infrared near-field microscopy to chemically map the morphology of biological matrices. The investigated sample is built up from surface-tethered membrane proteins (cytochrome c oxidase) reconstituted in a lipid bilayer. We have carried out infrared near-field measurements in the frequency range between 1600 and 1800 cm−1. By simultaneously recording the topography and chemical fingerprint of the protein-tethered lipid bilayer with a lateral resolution of 80 nm × 80 nm, we were able to probe locally the chemical signature of this membrane and to provide a local map of its surface morphology.

Graphical abstract: Scanning near-field IR microscopy of proteins in lipid bilayers

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Article information

DOI
https://doi.org/10.1039/C1CP21512D
Article type
Paper
Submitted
11 May 2011
Accepted
05 Oct 2011
First published
02 Nov 2011

Phys. Chem. Chem. Phys., 2011,13, 21432-21436

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Scanning near-field IR microscopy of proteins in lipid bilayers

F. Ballout, H. Krassen, I. Kopf, K. Ataka, E. Bründermann, J. Heberle and M. Havenith, Phys. Chem. Chem. Phys., 2011, 13, 21432 DOI: 10.1039/C1CP21512D

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