Issue 11, 2011

Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation

Abstract

DNA strands containing a 5-hydroxymethylcytosine (hmC), which have recently been found in neuron cells and embryonic stem cells, were synthesized through a facile synthetic technique. The hmC-containing strands were efficiently oxidized at hmC using an osmium oxidation assay. The hmC was oxidized as easily as 5-methylcytosine, which can be distinguished from unmethylated cytosine.

Graphical abstract: Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation

Supplementary files

Article information

Article type
Paper
Submitted
16 Feb 2011
Accepted
28 Mar 2011
First published
28 Mar 2011

Org. Biomol. Chem., 2011,9, 4176-4181

Facile synthesis of hydroxymethylcytosine-containing oligonucleotides and their reactivity upon osmium oxidation

K. Sugizaki, S. Ikeda, H. Yanagisawa and A. Okamoto, Org. Biomol. Chem., 2011, 9, 4176 DOI: 10.1039/C1OB05247K

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