Issue 8, 2012

Analysis of biomarkers characteristic of porcine liver injury—from biomolecular logic gates to an animal model

Abstract

A biocatalytic cascade for the analysis of the simultaneous increase in the concentration of two biomarkers characteristic of liver injury (alanine transaminase, ALT, and lactate dehydrogenase, LDH) was tested on real samples acquired from an animal model (domestic pigs, Sus scrofa domesticus) suffering from traumatic liver injury. A two-step reaction biocatalyzed in the presence of both enzyme-biomarkers resulted in the oxidation of NADH followed by optical absorbance measurements. A simple qualitative, YES/NO, test allowed for distinction between animals with and without the presence of liver injury with the probability of 92%. These data represent the first demonstration of applying binary logic systems for the analysis of real biomedical samples.

Graphical abstract: Analysis of biomarkers characteristic of porcine liver injury—from biomolecular logic gates to an animal model

Article information

Article type
Communication
Submitted
04 Jan 2012
Accepted
21 Feb 2012
First published
22 Feb 2012

Analyst, 2012,137, 1768-1770

Analysis of biomarkers characteristic of porcine liver injury—from biomolecular logic gates to an animal model

L. Halámková, J. Halámek, V. Bocharova, S. Wolf, K. E. Mulier, G. Beilman, J. Wang and E. Katz, Analyst, 2012, 137, 1768 DOI: 10.1039/C2AN00014H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements